Evaluating the physiological and hormonal responses of caper plant (Capparis spinosa) subjected to drought and salinity

Document Type: Research Paper

Authors

Department of Natural Resources and Environmental Engineering, College of Agriculture, Shiraz University, Shiraz, Iran

Abstract

In order to investigate the effect of drought and salinity stress on caper plant (Capparis spinosa), a factorial experiment including two factors consisting of drought and salinity levels based on randomized complete block design (RCBD) with five blocks were carried out at College of Agriculture, Shiraz University, Shiraz, Iran. After pretreatment, seeds were germinated in petri dishes and then transported to pots, to grow out of the glasshouse under natural conditions (same as environmental condition). Measuring morphological (Plant height, number of leaves, length of greatest leaf, root length, shoot fresh weight, shoot dry weight, root fresh weight and root dry weight, and chlorophyll (pad) and also physiological traits (relative water content (RWC) and water saturated deficit (WSD) for both root and shoot, root water content (RooWC) and leaf water content (LWC) showed that salinity and drought stresses as well as ABA concentration had negative effects on the plant. ABA content was significantly higher in salt-treated plants than in drought treated ones. The injury effect of salt stress was lower than drought. The results also indicated that the changes in response to the two stresses were low and so that this plant could be a suitable candidate for sowing and using in arid and semi-arid regions and also in saline condition. 

Keywords


Amjad M., J. Akhtar, M. Anwar-ul-Haq, A. Yang, S.S.    Akhtar, S.E. Jacobsen, 2014. Integrating role of ethylene and ABA in tomato plants adaptation to salt stress. Scientia Horticulture, 172; 109–116.

Antoni, R., L. Rodriguez, M. Gonzalez-Guzman, G.A. Pizzio, P.L. Rodriguez, 2011. News on ABA transport,
protein degradation, and ABFs/WRKYs in ABA signaling. Current Opinion. Plant Biology, 14; 1–7.

Auge, RM., H.D. Toler, A.M. Saxton, 2015. Arbusculr mycorrhizal symbiosis alter stomatal conductance of host plants more under drought than under amply watered conditions: a meta-analysis. Mycorrhiza, 25; 13-24.

Barrs, H., T. Kozlowski, 1968. Determination of water deficit in plant tissues. Water Deficit and Plant Growth, 1; 235-268.

Ben Hassine, A., S. Lutts, 2010. Differential responses of saltbush Atriplex halimus L. exposed to salinity and water stress in relation to senescing hormones abscisic acid and ethylene. Journal of Plant Physiology, 167; 1448–1456.

Claeke, J.M., T.N. McCaig, 1982. Excised leaf water retention capability as an indicator of drought resistance of Triticum  enotypes. Canadian Journal plant Science, 62; 571-578.

Hariadi, Y., K. Marandon, Y. Tian, S.E. Jacobsen, S. Shabala, 2011. Ionic and osmotic relations in quinoa (Chenopodium
     quinoa
Willd.) plants grown at various salinity levels. J. Experimental Botany, 62; 185–193.

Hu, X.L., M.Y. Jiang, J.H. Zhang, A.Y. Zhang, F. Lin, M.P. Tan, 2006. Calcium calmodulin is required for abscisic acid-induced antioxidant defense and functions both upstream and downstream of H2O2 production in leaves of maize (Zea mays) plants. New Phytolgist, 173; 27-38.

Javid, M.G., A. Sorooshzadeh, F. Moradi, S. Modarres, S. Sanavy, I. Allahdadi, 2011. The role of phytohormones in  alleviating salt stress in crop plants. Australian Journal of Crop Science; 5; 726–734.

Jiang, M., J. Zhang, 2002. Role of abscisic acid in water stress-induced antioxidant defense in leaves of maize seedlings. Free Radical Research, 36; 1001–1015.

Legua P., J. Martinez Melgarejo, R. Martinez, F. Hernandez, 2013. Phonological growth stages of caper plant (Capparis spinosa L.) according to the Biologische Bundesanstalt, Bundessortenamt and chemical scale. Annals of Applied Biology, 163; 135-141.

Lovelli S, A,Scopa Perniola,  T, Di Tommaso, A, Sofo, 2012. Abscisic acid root and leaf concentration in relation to biomass partitioning in salinized tomato plants. Journal of Plant Physiology, 169; 226– 233.

Mussalam, I., M. Duwayri, R. Shibi, F. Alali, 2012. Investigation of rutin content in different plant parts of wild caper (Capparis spinosa L.) populations from Jordan. Research Journal of Medicinal Plants, 6; 27-36.

Nemali, K.S., C. Bonin, F.G. Dohleman, M. Stephans, W.R. Reeves, D.E. Nelson, P. Castiglioni, J.E. Whitsel­Sammons R.A.  Silady, 2014. Physiological responses related to increased grain yield under drought in the first biotechnology –derived drought tolerant maize. Plant, cell & Environment, 38; 1866-1880.

Pessarakli, M., 2014. Handbook of plant and crop physiology. CRC Press.

Pirasteh Anosheh, H., H. Sadeghi, Y. Emam, 2011. Chemical Priming with Urea and KNO3 Enhances Maize Hybrids (Zea mays L.) Seed Viability under Abiotic Stress. Journal of Crop Science and Biotechnology, 14; 289–295.

Sadeghi, H., Z. Robati, 2015. Response of Cichorium intybus L. to eight seed priming methods under osmotic stress conditions. Biocatalysis and Agricultural Biotechnology, 4; 443-448  

Saed-Moocheshi, A., A. Shekoofa, H. Sadeghi, M. Pessarakli, 2014. Drought and salt stress mitigation by seed priming   with Kno3 and urea in various maize hybrids: an experimental approach based on enhancing antioxidant responses. Journal of Plant Nutrition, 37; 674-689.

Saifi, N., J, Ibijbijen, D. Echgadda, 2011. Genetic diversity of caper plant (Capparis spp) from north Morocco. Journal of food Agriculture Environment, 9; 14-20.

Suleiman, M.K.,  N.R.  Bhat,  S.  Jacob, R.R. Thomas, 2012. Effect of rooting hormones (IBA and NAA) on rooting of Semi hard wood cuttings of Capparis spinosa. Journal of Agriculture and Biodiversity, 1; 135-139.

Suzuki, N., R.M. Rivero Shulaev, E. Blumwald, R. Mittler, 2014. Abiotic and biotic stress combinations. New Phytologist, 203; 32-43.

Zhang, J., W. Jia, J. Yang, A.M. Ismail, 2006. Role of ABA integrating plant responses to drought and salt stresses. Field Crop Research, 97; 11–9.

Zhang, L.X., J.H. Lai, Z.S. Liang, M. Ashraf, 2014. Interactive effects of sudden and gradual drought stress and foliar-applied Glycinbetaine on growth, water relations, osmolyte accumulation and antioxidant defense system in two maize cultivars differing in drought tolerance. Journal of Agronomy and Crop Science, 200; 425-433.