Halopriming and Hydropriming Treatments to Overcome Salt and Drought Stress at Germination Stage of Corn (Zea mays L.)

Document Type: Research Paper


Shahrood University of Technology, Shahrood, Iran


To study the effects of halopriming and hydropriming in overcoming salt and drought stress in corn (Zea mays L.),
two experiments were separately conducted at Shahrood University of Technology. Seed treatments consisted of
control (untreated seeds), soaking in distilled water for 32 h (hydropriming), and soaking in 50 mmol solution of
CaCl2 for 16 h (halopriming). Germination and early seedling growth were studied using distilled water (control) and
osmotic potentials of -0.4, -0.8, and -1.2 MPa from NaCl (salinity stress) and polyethylene glycol [PEG 6000
(drought stress)]. Results showed that in both salinity and drought experiments, germination percentage reduced
significantly according to decreased osmotic potential. Hydroprimed and haloprimed seeds achieved the minimum
reductions in germination percentage. The maximum reduction in germination percentage was recorded from
untreated seeds (control). Minimum reduction percentages of root length (RPL root) and shoot length (RPL shoot)
were attained from hydroprimed and haloprimed seeds due to NaCl and PEG conditions (-0.4 MPa), and maximum
RPL root and RPL shoot were attained from controlled seeds due to NaCl and PEG (-1.2 MPa) conditions. The
reduction percentage of dry weight for root (RPD root) and shoot (RPD shoot) increased according to increased
osmotic potential in both NaCl and PEG, but RPD for shoot was significantly affected compared with RPD for root.
Interaction of seed priming treatment and osmotic potential for the germination index (GI) showed that under 0 and -
0.4 MPa, hydroprimed and haloprimed seeds had higher GI as compared with untreated seeds due to NaCl and PEG
conditions. Interaction between the seed priming treatment and osmotic potential significantly affected the vigour
index (VI) due to NaCl and PEG conditions, and halopriming significantly increased VI at high osmotic potentials.
On average, the VI of haloprimed seeds was higher than that of untreated seeds at high osmotic potentials and was
not significantly different from hydroprimed and untreated seeds at low osmotic potentials. It is concluded that under
salinity stress, the osmotic effect is more important than the toxic effect in loss of seed germination. Moreover,
hydropriming practically ensured rapid and uniform germination with few abnormal seedlings.


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