The effect of vinasse as a carbon source on the activity of urease-producing bacteria in the microbially induced calcite precipitation (MICP) approach.

Document Type : Research Paper

Authors

1 Department of Soil Science, Agricultural Sciences and Natural Resources university of Khuzestan, Iran

2 Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 Biotechnology and Biological Science Research Center, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Abstract

One of the most important causes of soil desertification is wind erosion. Deserts are known for their poor physical, chemical, and biological properties. In order to solve the problem of loose sand, many solutions have been thought and used so far. Today, scientists have greatly benefited from the capabilities of MICP in the field of sand dune stabilization, which seems to be an environmentally friendly solution. In Khuzestan, a lot of "vinasse" enter the environment every year, which is considered a pollutant. Combining it with beneficial native microorganisms can turn it into a valuable ingredient. The objectives of this study were to investigate the microbial activity of bacteria isolated from the sand dunes of Khuzestan in Iran using vinasse as a carbon source for growth. In order to eliminate the effect of competition and investigate the activity of the studied bacterial isolates (Bacillus licheniformis MZ057843, MZ057842, OP329211, and Sporosarcina pasteurii), the samples of sand dunes collected from Khuzestan were first sterilized. Then, inoculated vinasses were added to them, and the prepared samples were incubated for five weeks. The experiments (counting bacteria by plate count technique, Soil catalase by the method of titration with KMnO4, and Soil microbial respiration by measuring the amount of CO2) were measured after the first, third and fifth weeks of incubation time. Then, the data were analyzed by split plot in time design. The current study showed significant effects of vinasse, bacteria, time and their interaction in all three tests (p < 0.0001). Isolate B1 was able to cause the highest amount of microbial population and microbial respiration, and isolate B3 produced the highest amount of catalase enzyme in the soil. Among the three types of vinasse, V3 had the highest amount of catalase and microbial population, and V2 had the highest soil microbial respiration rate. The best incubation time for the microbial respiration and population tests was the first week, and third week of incubation for the catalase test. Generally, native Bacillus bacteria grew in vinasse as a carbon source and perhaps the potential of vinasse as a mulch can be explored. However, more research is needed to use it in soil stabilization operations.

Keywords

References

 
Abdelhamid, M. A., A. M. Meligy, K. B. Yeo, C. S. Lee, S. P. Pack, 2020. Silaffin-3-derived pentalysine cluster as a new fusion tag for one-step immobilization and purification of recombinant Bacillus subtilis catalase on bare silica particles. International journal of biological macromolecules, 159; 1103-1112.
Alves, P. R. L., G. A. Estrada-Bonilla, D. Bini, E. J. B. N. Cardoso, 2019. Changes in the microbial metabolism of agricultural tropical soils amended with sugarcane vinasses. Sugar Tech, 21; 364-369.
Azoogh, L., S. Jafari, 2018. Interaction of petroleum mulching, vegetation restoration and dust fallout on the conditions of sand dunes in southwest of Iran. Aeolian research, 32; 124-132.
Baker, C. N., C. Thornsberry, R. W. Hawkinson, 1983. Inoculum standardization in antimicrobial susceptibility testing: evaluation of overnight agar cultures and the rapid inoculum standardization system. Journal of clinical microbiology, 17(3); 450-457.
Balakrishnan, D. 2024. Exploring the potential of sugarcane vinasse for biogas and biofertilizer Production: A catalyst for advancing the bioeconomy. Sustainable Energy Technologies and Assessments, 61; 103474.
Buckley, S., R. Brackin, T., Näsholm, S. Schmidt, S. Jämtgård, 2022. The influence of sucrose on soil nitrogen availability–A root exudate simulation using microdialysis. Geoderma, 409; 115645.
Chabot, M., E. Morales, J. Cummings, N. Rios, S. Giatpaiboon, R. Mogul, 2020. Simple kinetics, assay, and trends for soil microbial catalases. Analytical biochemistry, 610; 113901.
Cho, W. I., M. S. Chung, 2020. Bacillus spores: A review of their properties and inactivation processing technologies. Food science and biotechnology, 29; 1447-1461.
Christofoletti, C. A., J. P. Escher, J. E. Correia, J. F. U. Marinho, C. S. Fontanetti, 2013. Sugarcane vinasse: environmental implications of its use. Waste management, 33(12); 2752-2761.
Chu, D., N. Ilyas, L. Peng, X. Wang, D. Wang, Z. Xu, Q. Gao, X. Tan, C. Zhang, Y. Li, Y. Yuan, 2022. Genomic insights on fighting bacterial wilt by a novel Bacillus amyloliquefaciens strain Cas02. Microbial Biotechnology, 15(4); 1152-1167.
Cordoba, A. S., M. M. de Mendonça, S. L. Stürmer, P. T. Rygiewicz, 2001. Diversity of arbuscular mycorrhizal fungi along a sand dune stabilization gradient: A case study at Praia da Joaquina, Ilha de Santa Catarina, South Brazil. Mycoscience, 42(4); 379-387.
Cruz-Paredes, C., Tájmel, D., & Rousk, J. (2021). Can moisture affect temperature dependences of microbial growth and respiration? Soil Biology and Biochemistry, 156; 108223.
de Carvalho, J. C., L. P. de Souza Vandenberghe, E. B. Sydney, S. G. Karp, A. I.  Magalhães, W. J. Martinez-Burgos, A. B. P. Medeiros, V. Thormaz-Soccol, S. Vieira, L. A. J. Letti, C. Rodriguez, A. l. Woiciechowski, C. R. Soccol, 2023. Biomethane Production from Sugarcane Vinasse in a Circular Economy: Developments and Innovations. Fermentation, 9(4); 349.
Dlamini, P. J. 2021. Drought stress tolerance mechanisms and breeding effort in sugarcane: A review of progress and constraints in South Africa. Plant Stress, 2; 100027.
Duquenne, P., C. Chenu, G. Richard, G. Catroux, 1999. Effect of carbon source supply and its location on competition between inoculated and established bacterial strains in sterile soil microcosm. FEMS microbiology ecology, 29(4); 331-339.
Feng, J., B. Chen, W. Sun, Y. Wang, 2021. Microbial induced calcium carbonate precipitation study using Bacillus subtilis with application to self-healing concrete preparation and characterization. Construction and Building Materials, 280; 122460.
Ferreira, L. F. R., N. H. Torres, R. D. de Armas, C. D. Fernandes, D. da Silva Vilar, M. M. Aguiar, G. B. Pompeo, R. T. R. Monteiro, H. M. N. Iqbal, M. Bilal, R. N. Bharagava, 2020. Fungal lignin-modifying enzymes induced by vinasse mycodegradation and its relationship with oxidative stress. Biocatalysis and Agricultural Biotechnology, 27; 101691.
Fulbright, T. E., J. A. Ortega-Santos, A. Lozano-Cavazos, L. E. Ramírez-Yanez, 2006. Establishing vegetation on migrating inland sand dunes in Texas. Rangeland Ecology & Management, 59(5); 549-556.
Ghorbanzadeh, N., H., Pourbabaei, A. Salehi, T. A. A., Soltani, S. J. ALAVI, 2018. Investigation of microbial diversity and soil microorganisms in softwood and hardwood plantations in the west of Guilan province. Applied Soil Research, 6(3); 1-12. (in Persian)
Ghorbanzadeh, N., M. B. Farhangi, 2021. Effect of Agro-industrial Solid Waste and Bacillus and Pseudomonas Bacteria on Ecophysiological Indices in a Loam Soil. Journal of Soil Management and Sustainable Production, 10(4); 31-54. (in Persian)
Gong, X. W., J. J. Guo, D. M. Jiang, X. H. Li, F. G. Scholz, S. J. Bucci, G. Goldstein, G. Y. Hao, 2020. Contrasts in xylem hydraulics and water use underlie the sorting of different sand-fixing shrub species to early and late stages of dune stabilization. Forest Ecology and Management, 457; 117705.
Gowthaman, S., S. Mitsuyama, K. Nakashima, M. Komatsu, S. Kawasaki, 2019. Biogeotechnical approach for slope soil stabilization using locally isolated bacteria and inexpensive low-grade chemicals: A feasibility study on Hokkaido expressway soil, Japan. Soils and foundations, 59(2); 484-499.
Grigatti, M., L. Barbanti, C. Ciavatta, 2010. Soil respiration and nitrogen mineralization kinetics of compost and vinasse fertilized soil in an aerobic liquid-based incubation. Environmental Engineering Science, 27(1); 65-73.
Guangming, L., Z. Xuechen, W. Xiuping, S. Hongbo, Y. Jingsong, W. Xiangping, 2017. Soil enzymes as indicators of saline soil fertility under various soil amendments. Agriculture, Ecosystems & Environment, 237; 274-279.
Gumaa, Y. T., I. Haffar, M. A. Al-Afifi, 1998. Financial appraisal of date-frond mat fence systems for wind erosion control and sand dune stabilization in the arid region of the United Arab Emirates. Journal of arid environments, 39(4); 549-557.
Hale, L., M. Luth, R. Kenney, D. Crowley, 2014. Evaluation of pinewood biochar as a carrier of bacterial strain Enterobacter cloacae UW5 for soil inoculation. Applied Soil Ecology, 84; 192-199.
Hashemimanesh, M., H. Matinfar, 2012. Evaluation of desert management and rehabilitation by petroleum mulch base on temporal spectral analysis and field study (case study: Ahvaz, Iran). Ecological Engineering, 46; 68-74.
Hashempour, E., M. B. Farhangi, N. Ghorbanzadeh, M. F. Sangani, 2020. The effect of Bacillus bacteria on phosphorus release from oil refinery plant-solid waste in a calcareous soil. Journal of Water and Soil, 34(1); 129-143. (in Persian)
He, H., Q. Yu, Z. Ding, L. Zhang, G. Shi, Y. Li, 2023. Biotechnological and food synthetic biology potential of platform strain: Bacillus licheniformis. Synthetic and Systems Biotechnology, 8(2); 281-291.
Huang, M., C. Lee, S. Ho, K. Lin, C. Pan, 2013. High-yield levan produced by Bacillus licheniformis FRI MY-55 in high-sucrose medium and its prebiotic effect. Journal of Pure and Applied Microbiology, 7(3); 1585-1599.
Jafari, S., M. Chorom, N. Enayatizamir, H. Motamedi, 2013. Evaluating the effects of Bacillus subtilis and corynebacterium glutamicum on soil microbial indexes in different levels of salinity. Journal of Agricultural Engineering Soil Science and Agricultural Mechanization. Scientific Journal of Agriculture, 35(2); 55-70. (in Persian)
Jamili, T., B. Khalilimoghadam, E. Shahbazi, 2015. Investigation of water holding capacity of sugarcane mulch for sand dune stabilization in ahvaz. Water and Soil, 29(5); 1278-1287 (in Persian).
Johnson, J. L., K. L. Temple, 1964. Some variables affecting the measurement of “catalase activity” in soil. Soil Science Society of America Journal, 28(2); 207-209.
Jozedaemi, E., A. Golchin, 2024. Changes in aggregate-associated carbon and microbial respiration affected by aggregate size, soil depth, and altitude in a forest soil. Catena, 234; 107567.
Kelliher, F. M., M. M. Barbour, J. E. Hunt, 2005. Sucrose application, soil microbial respiration and evolved carbon dioxide isotope enrichment under contrasting land uses. Plant and Soil, 268; 233-242.
Khalili Moghadam, B., T. Jamili, H. Nadian, E. Shahbazi, 2016. The influence of sugarcane mulch on sand dune stabilization in Khuzestan, the southwest of Iran. Iran Agricultural Research, 34(2); 71-80.
Kim, G., J. Kim, H. Youn, 2018. Effect of temperature, pH, and reaction duration on microbially induced calcite precipitation. Applied Sciences, 8(8); 1277.
Klaewkla, M., R. Pichyangkura, T. Charoenwongpaiboon, K. Wangpaiboon, S. Chunsrivirot, 2020. Computational design of oligosaccharide producing levansucrase from Bacillus licheniformis RN-01 to improve its thermostability for production of levan-type fructooligosaccharides from sucrose. International Journal of Biological Macromolecules, 160; 252-263.
Lakra, U., V. Lincoln Singh Munda, V. K. Nigam, & S. R. Sharma, 2023. Optimisation, characterization, and biological evaluation of novel exopolysaccharide from Bacillus licheniformis (BITSL006). Natural Product Research, 1-10.
Li, Q., J. H. Liang, Y. Y. He, Q. J. Hu, S. Yu, 2014. Effect of land use on soil enzyme activities at karst area in Nanchuan, Chongqing, southwest China. Plant Soil Environ, 60(1); 15-20.
Li, Y., Z. Guo, L. Wang, H. Yang, 2023. A coupled bio-chemo-hydro-wave model and multi-stages for MICP in the seabed. Ocean Engineering, 280; 114667.
Maleki, M., S. Ebrahimi, F. Asadzadeh, M. Emami Tabrizi, 2016. Performance of microbial-induced carbonate precipitation on wind erosion control of sandy soil. International journal of environmental science and technology, 13; 937-944.
Micallef, S. A., M. T. Callahan, R. McEgan, L. Martinez, 2023. Soil Microclimate and Persistence of Foodborne Pathogens Escherichia coli O157: H7, Listeria Monocytogenes, and Salmonella Enterica Newport in Soil Affected by Mulch Type. Journal of Food Protection, 86(11); 100159.
Nasser, A. A., N. M. Sorour, M. A. Saafan, R. N. Abbas, 2022. Microbially-Induced-Calcite-Precipitation (MICP): A biotechnological approach to enhance the durability of concrete using Bacillus pasteurii and Bacillus sphaericus. Heliyon, 8(7).
Ni, J., S. Tokuyama, A. Sogabe, Y. Kawamura, Y. Tahara, 2001. Cloning and high expression of catalase gene from Bacillus sp. TE124. Journal of bioscience and bioengineering, 91(4); 422-424.
Ni, J., Y. Sasaki, S. Tokuyama, A. Sogabe, Y. Tahara, 2002. Conversion of a typical catalase from Bacillus sp. TE124 to a catalase-peroxidase by directed evolution. Journal of bioscience and bioengineering, 93(1); 31-36.
Omeroglu, M. A., M. BaltaciTaskin, A. Adiguzel, 2024. Turning waste into biofuel: Non-sterile fermentation of molasses using immobilized cells of Bacillus licheniformis BD43 for efficient 2, 3-Butanediol production. Industrial Crops and Products, 211; 118265.
Paar, A., S. Costa, T. Tzanov, M. Gudelj, K. H. Robra, A. Cavaco-Paulo, G. M. Gübitz, 2001. Thermo-alkali-stable catalases from newly isolated Bacillus sp. for the treatment and recycling of textile bleaching effluents. Journal of biotechnology, 89(2-3); 147-153.
Parkin, T. B., J. W. Doran, E. Franco-Vizcaíno, 1997. Field and laboratory tests of soil respiration. Methods for assessing soil quality, 49; 231-245.
Qin, S. K., Z. C. Cui, H. Li, X. F. Zeng, R. Q. Zhang, C. M. Yang, 2023. Effects of Bacillus licheniformis on growth performance, immune and antioxidant functions and intestinal microbiota of yellow-feathered broilers. Chinese Journal of Animal Nutrition, 35(2); 865-873.
San Pablo, A. C., M. Lee, C. M. Graddy, C. M. Kolbus, M. Khan, A. Zamani, N. Martin, C. Acuff, J. T. DeJong, M. G. Gomez, D. C. Nelson, 2020. Meter-scale biocementation experiments to advance process control and reduce impacts: Examining spatial control, ammonium by-product removal, and chemical reductions. Journal of Geotechnical and Geoenvironmental Engineering, 146(11); 04020125.
Schinner, F., R. Öhlinger, E. Kandeler, R. Margesin, 2012. Methods in soil biology. Springer Science & business media, Springer-Verlag Berlin Heidelberg New York.
Sen, A., M. Ozkarsli, N. M. Dogan, A. Semiz, S. Arslan, 2011. Cloning, expression, purification and characterization of Bacillus licheniformis catalase from Pamukkale Hot Springs. Current Opinion in Biotechnology, (22); S39.
Senatore, D., A. Queirolo, J. Monza, N. Bajsa, 2023. Using sugar cane vinasse as a biofertilizer: effects on soil microbial community due to periodical application. Environmental Sustainability, 6(2); 173-182.
Shaeer, A., M. Aslam, N. Rashid, 2021. Structural and functional analyses of a novel manganese-catalase from Bacillus subtilis R5. International Journal of Biological Macromolecules, 180; 222-233.
Sharma, M., R. Reynnells, 2016. Importance of soil amendments: Survival of bacterial pathogens in manure and compost used as organic fertizliers. Microbiology Spectrum, 4; 1-13.
Shi, M., Y. Kang, W. Zhang, X. Yang, Y. Fan, H. Yu, R. Zhang, A. Gou, S. Qin, 2022. Plastic film mulching with ridge planting alters soil chemical and biological properties to increase potato yields in semiarid Northwest China. Chemical and Biological Technologies in Agriculture, 9(16); 1-12.
Silva, M. T., F. Gasparotto, B. M. Lustri, N. C. Vasques, N. U. Yamaguchi, 2020. Cultivation of Azospirillum brasilense in vinasse and potential use in fertigation. Journal of Agricultural Studies, 8(4); 726-734.
Soto, M. F., C. A. Diaz, A. M. Zapata, J. C. Higuita, 2021. BOD and COD removal in vinasses from sugarcane alcoholic distillation by Chlorella vulgaris: Environmental evaluation. Biochemical Engineering Journal, 176; 108191.
Sun, X., L. Miao, L. Wu, 2020. Applicability and theoretical calculation of enzymatic calcium carbonate precipitation for sand improvement. Geomicrobiology journal, 37(4); 389-399.
Tang, C. S., L. Y. Yin, N. J. Jiang, C. Zhu, H. Zeng, H. Li, B. Shi, 2020. Factors affecting the performance of microbial-induced carbonate precipitation (MICP) treated soil: a review. Environmental Earth Sciences, 79; 1-23.
Tejada, M., A. M. García-Martínez, J. Parrado, 2009. Effects of a vermicompost composted with beet vinasse on soil properties, soil losses and soil restoration. Catena, 77(3); 238-247.
Torres, M. A., A. L. Valdez, M. V. Angelicola, E. E. Raimondo, H. F. Pajot, C. G. Nieto-Peñalver, 2022. Cultivation of plant-growth promoters in vinasse: contributions for a circular and green economy. bioRxiv, 2022-12.
Torres, M. A., A. L. Valdez, M. V. Angelicola, E. E. Raimondo, H. F. Pajot, C. G. Nieto-Peñalver, 2023. Vinasse as a substrate for inoculant culture and soil fertigation: Advancing the circular and green economy. Science of The Total Environment, 887; 164014.
Trasar-Cepeda, C., F. Camiña, M. C. Leirós, F. Gil-Sotres, 1999. An improved method to measure catalase activity in soils. Soil Biology and Biochemistry, 31(3); 483-485.
Trevors, J. T. 1984. Rapid gas chromatographic method to measure H2O2 oxidoreductase (catalase) activity in soil. Soil Biology and Biochemistry, 16 (5); 525-526.
Vacek, Z., D. Řeháček, J. Cukor, S. Vacek, T. Khel, R. P. Sharma, J. Cusera, J. Kral, V. Papaj, 2018. Windbreak efficiency in agricultural landscape of the Central Europe: multiple approaches to wind erosion control. Environmental management, 62; 942-954.
Vaclavik, E., B. Halling-Sørensen, F. Ingerslev, 2004. Evaluation of manometric respiration tests to assess the effects of veterinary antibiotics in soil. Chemosphere, 56(7); 667-676.
Vail, M., C. Zhu, C. S. Tang, N. Maute, M. T. Montalbo-Lomboy, 2020. Desiccation cracking behavior of clayey soils treated with biocement and bottom ash admixture during wetting–drying cycles. Transportation Research Record, 2674(8); 441-454.
Van Paassen, L. A., C. M. Daza, M. Staal, D. Y. Sorokin, W. van der Zon, M. C. van Loosdrecht, 2010. Potential soil reinforcement by biological denitrification. Ecological Engineering, 36(2); 168-175.
Wall, D. H., R. A. Virginia, 1999. Controls on soil biodiversity: insights from extreme environments. Applied Soil Ecology, 13(2); 137-150.
Wang Y. D., M. YunChuan, W. WeiHao, L. YangRui, L. Y., Y. YanPing, 2006. Effect of vinasse irrigation on the activity of three enzymes and agronomic characters at seedling stage of sugarcane. Sugar Tech, 8; 264–267
Wang, X., J. Zhu, H. Wei, Z. Ding, X. Li, Z. Liu, H. Wang, Y. Wang, 2023. Biological control efficacy of Bacillus licheniformis HG03 against soft rot disease of postharvest peach. Food Control, 145; 109402.
Wang, Y., D. Niu, X. Yuan, D. Guo, H. Fu, J. J. Elser, 2023. Dominant plant species alter stoichiometric imbalances between soil microbes and their resources in an alpine grassland: Implications for soil microbial respiration. Geoderma, 431; 116336.
Wang, Y., K. Yang, Z. Tang, 2019. In situ effect of combined utilization of fly ash and polyacrylamide on sand stabilization in North China. Catena, 172; 170-178.
Wu, D., T. Xia, Y. Zhang, Z. Wei, F. Qu, G. Zheng, S. Song, Y. Zhao, K. Kang, H. Yang, 2021. Identifying driving factors of humic acid formation during rice straw composting based on Fenton pretreatment with bacterial inoculation. Bioresource Technology, 337; 125403.
Xie, X., Z. He, N. Chen, Z. Tang, Q. Wang, Y. Cai, 2019. The roles of environmental factors in regulation of oxidative stress in plant. BioMed research international, 1-11.
Xu, A., R. L. Buchanan, S. A. Micallef, 2016. Impact of mulches and growing season on indicator bacteria survival during lettuce cultivation. International Journal of Food Microbiology, 224; 28-39.
Xu, H., Z. Li, L. Li, X. Xie, D. Cai, Z. Wang, Y. Zhan, S. Chen, 2023. Sustainable production of 2-phenylethanol from agro-industrial wastes by metabolically engineered Bacillus licheniformis. LWT, 173; 114414.
Xu, Y., Y. Yu, Y. Shen, Q. Li, J. Lan, Y. Wu, R. Zhang, G. Cao, C. Yang, 2021. Effects of Bacillus subtilis and Bacillus licheniformis on growth performance, immunity, short chain fatty acid production, antioxidant capacity, and cecal microflora in broilers. Poultry science, 100(9); 101358.
Yuan, F., S. Yin, Y. Xu, L. Xiang, H. Wang, Z. Li, K. Fan, G. Pan, 2021. The richness and diversity of catalases in bacteria. Frontiers in Microbiology, 12; 645477.
Zehner, J., A. Røyne, A. Wentzel, P. Sikorski, 2020. Microbial-induced calcium carbonate precipitation: an experimental toolbox for in situ and real time investigation of micro-scale pH evolution. RSC advances, 10(35); 20485-20493.
Zhang, T. H., H. L. Zhao, S. G. Li, F. R. Li, Y. Shirato, T. Ohkuro, I. Taniyama, 2004. A comparison of different measures for stabilizing moving sand dunes in the Horqin Sandy Land of Inner Mongolia, China. Journal of Arid Environments, 58(2); 203-214.
Zheng, Y., X. Han, Y. Li, J. Yang, N. Li, N. An, 2019. Effects of biochar and straw application on the physicochemical and biological properties of paddy soils in northeast China. Scientific reports, 9(1); 16531.
Zhou, J., Y. Wu, D. Liu, R. Lv, 2023. The effect of carbon source and temperature on the formation and growth of Bacillus licheniformis and Bacillus cereus biofilms. LWT, 186; 115239.
Zuo, X., H. Zhao, X. Zhao, T. Zhang, Y. Guo, S. Wang, S. Drake, 2008. Spatial pattern and heterogeneity of soil properties in sand dunes under grazing and restoration in Horqin Sandy Land, Northern China. Soil and Tillage Research, 99(2); 202-212.
 
 
Desert
Volume 29, Issue 1
June 2024
Pages 53-70

Files

Share

How to cite

Statistics