As an herbaceous, perennial, and evergreen plant, Vetiver Grass (Chrysopogon zizanioides L.) can be used for the improvement and development of arid and semi-arid rangelands. To assess the interaction effect of organic and biological fertilizers on the growth and physiological traits of Vetiver grass, the dry weight of shoots and roots, essential oils, chlorophyll, carotenoids, and proline content were measured. A completely randomized design in factorial layout with three replications was performed in Malayer University’s greenhouse in 2016. The treatments were mycorrhizal fungi on two inoculated (M1) and non-inoculated (M2) levels and vermicompost organic fertilizer in six levelsof (0) (control), 10, 20, 40, 60, and 80% (V1, V2, V3, V4, V5 and V6), which were added to each pot. The results showed that the interaction between different levels of vermicompost and mycorrhiza had a significant (P<0.05) effect on quantitative and qualitative traits of the plants. According to the results, the highest leaf dry weight was seen in VC6M1 treatment and the highest root dry weight wasobserved in VC5M2 and VC6M2 treatments. The highest percentage of essential oil content was observed in VC2M1 and VC5M2 treatments. It was also observed that in the control level of vermicompost and mycorrhizae treatment (VC1M2), the presence of proline index rose to its highest level. The maximum amount of chlorophyll a and carotenoid was seen in VC3M2treatment while the maximum percentage of chlorophyll b was observed in VC2M2 treatment.